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(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 <t>HPLC</t> column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.
Hplc Pump Postnova Analytics Pn1122, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 HPLC column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.

Journal: Biochemistry

Article Title: Oligomeric State of the Oxalate Transporter, OxlT †

doi: 10.1021/bi201175y

Figure Lengend Snippet: (A) 50-100 μg of OxlT was loaded onto a Shodex kw-803 HPLC column in the presence of 5% glycerol, 50 mM potassium MOPS, 100 mM potassium oxalate and 0.1% DDM (pH 7). The tracings show normalized outputs of detectors for measurement of A280 (UV, black), Light Scattering (LS, red) and Refractive Index (RI, blue). In a parallel trial in the same experiment and using the same buffer conditions, the instrument calibration constant K1 was obtained using a reference set of six protein standards of mass between 29 kDa and 141 kDa (see Experimental Procedures). (B) For illustrative purposes, using data from Part A, OxlT mass (red trace) was calculated for the region across the A280 trace at ca. 75% peak height. For this and other experiments, the mass values reported in Tables ​Tables11 and ​and22 were calculated at the peak of the A280 trace.

Article Snippet: Molecular weight determination by TD-SEC-LS Analytical size exclusion chromatography was conducted using a Postnova Analytics PN1122 HPLC pump in conjunction with a Shodex KW-G guard column in line with a Shodex KW-803 size exclusion column.

Techniques: Refractive Index